IL4 Receptor antibody (Extracellular Domain)

Catalog No. ABIN112606

Product Details anti-IL4 Receptor Antibody

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Binding Specificity: Extracellular Domain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL4 Receptor antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Enzyme Immunoassay (EIA)

Specificity: This antibody is specific for Interleukin 4 Receptor, the transmembrane ligand-binding subunit of the heterodimeric IL-4 receptor complex. This antibody blocks cell surface IL-4 receptor mediated bioactivities induced by IL-4 or IL-13. An ND50 was observed at approximately 5-20 ng/mL in the presence of 0.2 ng/mL of IL-4 using the factor dependant Human erythroleukemic cell line, TF-1.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography on Protein A

Immunogen: Sf 21-derived, recombinant Human interleukin 4 Receptor, extracellular domain.

Clone: 25463 (49-20)

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 1/20 - 1/80. ELISA: 1/500 - 1/2000. Western Blot: 1/250 - 1/1000, Will detect human IL-4 R under non-reducing conditions. Functional Assays. Immunohistochemistry on frozen sections: 1/40 - 1/125.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide, 10 % Trehalose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IL4 Receptor

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Alternative Name: CD124 / IL4R (IL4R Products)

Background: IL4 Receptor alpha (IL4RA) is the alpha chain of the interleukin 4 receptor which binds to both interleukin 4 and interleukin 13 to regulate IgE production, chemokine and mucus production at sites of allergic inflammmation. The IL4 response is involved in promoting Th2 differentiation. The secreted extracellular domain of IL4R alpha, called sIL4R alpha, can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. It has no signaling abilities. Allelic variations in the IL4RA gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. IL-4 R is expressed by B cells, T cells, monocytes and macrophages.Synonyms: 582J2.1, IL-4R-alpha, IL4 Receptor alpha, IL4RA, Interleukin-4 receptor alpha chain

Gene ID: 3566

NCBI Accession: NP_000409

UniProt: P24394

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process