TNC antibody

Catalog No. ABIN112483

Product Details anti-TNC Antibody

Target: TNC (Tenascin C (TNC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNC antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: The antibody recognizes tenascin, a large hexameric extracellular matrix glycoprotein.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein A.

Immunogen: Protein preparation from a homogenate of a human mammary tumour specimen

Clone: T2H5

Isotype: IgG1

Application Details

Application Notes: ELISA. Western Blotting. Immunoprecipitation. Immunohistochemistry on Paraffin Sections: Immunohistochemical detection of Tenascinis valuable for studies of tissue differentiation and tumour growth. The antibody isexcellent for staining of paraffin-embedded tissue sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-TNC antibody (ABIN112483)

Gulubova: "Immunohistochemical localization of collagen type III and type IV, laminin, tenascin and alpha-smooth muscle actin (alphaSMA) in the human liver in peliosis." in: Pathology, research and practice, Vol. 198, Issue 12, pp. 803-12, (2003) (PubMed).

Faustino, van Garderen, Schalken, Nederbragt: "Tenascin expression in normal, hyperplastic, dysplastic and neoplastic canine mammary tissues." in: Journal of comparative pathology, Vol. 126, Issue 1, pp. 1-8, (2002) (PubMed).

Ioachim, Charchanti, Briasoulis, Karavasilis, Tsanou, Arvanitis, Agnantis, Pavlidis et al.: "Immunohistochemical expression of extracellular matrix components tenascin, fibronectin, collagen type IV and laminin in breast cancer: their prognostic value and role in tumour invasion and ..." in: European journal of cancer (Oxford, England : 1990), Vol. 38, Issue 18, pp. 2362-70, (2002) (PubMed).

Bártek, Viklický, Fran?k, Angelisová, Dráber, Jarosíková, N?mec, Verlová: "Monoclonal antibodies against transferrin. Precipitating mixtures and lack of inter-species cross-reactivity." in: Immunology letters, Vol. 4, Issue 5, pp. 231-5, (1982) (PubMed).

Target Details for TNC

Target: TNC (Tenascin C (TNC))

Alternative Name: Tenascin / TNC / HXB (TNC Products)

Background: Tenascin is a high molecular ECM glycoprotein (MW app. 210-300 kDa), which contains hexamere repeat-rich single chains. It contains EGF-, Fibronectin III- and Fibronectin-similar structures. During organogenesis it is exprimed temporarily by many organs. During adulthood it reappears only in regeneration, wound healing and in stroma of various tumours. It probably has an anti-adhesive function, which plays a role during tumour speading. Tenascin is found in the central and peripheral nerve system, in smooth muscle, tendon and hyper-proliferative skin.Synonyms: Cytotactin, GMEM, GP 150-225, Glioma-associated-extracellular matrix antigen, Hexabrachion, Myotendinous antigen, Neuronectin, TN-C, Tenascin-C

Gene ID: 3371

NCBI Accession: NP_002151

UniProt: P24821

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development