Plakophilin 3 antibody (N-Term)

Catalog No. ABIN112378

Product Details anti-Plakophilin 3 Antibody

Target: Plakophilin 3 (PKP3)

Binding Specificity: AA 570-589, N-Term

Reactivity: Human, Mouse, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Plakophilin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody 23E3/4 reacts with an epitope located between residues 570-589 in Human Plakophilin-3.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse and Canine (Dog).

Purification: Purified

Immunogen: Synthetic peptide corresponding to amino acid residues 570-589 of Human Plakophilin-3 coupled to Keyhole Limpet Hemocyanin via an additional cysteine residue at the N- terminus (CFTPQSRRLRELPLAADALTF).

Clone: 23E3-4

Isotype: IgG2b

Application Details

Application Notes: This antibody is suitable for Immunocytochemistry (methanol-and paraformaldehyde-fixedcells), Immunohistochemistry on frozen and paraffin embedded tissues,Immunoprecipitation and Western blot detection. For optimal results, fixed cells and frozen tissues should be treated with 0.2 % Triton-X 100for, respectively, 5 min and 15 min prior to incubation with primary antibody. Recommended dilutions: Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) asdetection reagent: 1/25-1/200. Immunoblotting: 1/100-1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS with 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for Plakophilin 3

Target: Plakophilin 3 (PKP3)

Alternative Name: Plakophilin 3 / PKP3 (PKP3 Products)

Background: Plakophilin-3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells [1]. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin- 3 can be detected along cell borders in a punctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalisation, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin- 3 displays a dual intracellular localisation in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The human protein has a predicted molecular mass of 87 kD.Synonyms: Plakophilin-3

Gene ID: 11187

NCBI Accession: NP_009114

UniProt: Q9Y446