OB Cadherin antibody (Extracellular Domain)

Catalog No. ABIN112366

Product Details anti-OB Cadherin Antibody

Target: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Binding Specificity: Extracellular Domain

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This OB Cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Specificity: This antibody recognizes the extracellular domain of Cadherin-11.

Purification: Affinity Chromatography

Immunogen: 16A is a mouse monoclonal IgG1 antibody obtained by fusion of SP2/0 Mouse myeloma cells with spleen cells from a Mouse immunized with affinity purified extracellular domain of Human Cadherin-11-GST fusion protein.

Clone: 16A

Isotype: IgG1

Application Details

Application Notes: Suitable for Immunoblotting, Immunocytochemistry and Immunohistochemistry on Frozensections when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. Recommended Dilutions: 1/25-1/50 for Immunohistochemistry with Avidin-biotinylatedHorseradish Peroxidase Complex (ABC) as detection reagent.1/25-1/250 for Immunoblotting applications.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS with 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the original vial at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for OB Cadherin

Target: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Alternative Name: Cadherin-11 (CDH11 Products)

Background: Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. The progression of carcinomas is associated with the loss of epithelial morphology and a concomitant acquisition of a more mesenchymal phenotype, which is thought to contribute to the invasive and/or metastatic behavior. A putative role for cadherin-11 in these late stages of tumor progression is based on the fact that migration of mesenchymal cells is facilitated when cadherin-11 is highly expressed.Synonyms: CDH11, OB Cadherin, OSF-4, Osteoblast cadherin

Gene ID: 1009

NCBI Accession: NP_001788

UniProt: P55287