SYNPO antibody

Catalog No. ABIN112223

Product Details anti-SYNPO Antibody

Target: SYNPO (Synaptopodin (SYNPO))

Reactivity: Human, Rat, Mouse, Cow, Gerbil, Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This SYNPO antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: The antibody reacts specifically with Synaptopodin. It recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-Actinin. Does not react with parietal cells. Reacts with a subset of exclusively telencephalic synapses. Differentiation dependent expression during postnatal maturation of Rat brain. Differentiation dependent expression in cultured hippocampal neurons.

Cross-Reactivity (Details): Species reactivity (tested):Human, Bovine, Rat, Mouse, Guinea Pig and Gerbil. Negative with Rabbit, Frog and Chicken.

Purification: Affinity chromatography on Protein A

Immunogen: Isolated Rat kidney glomeruli.

Clone: G1D4

Isotype: IgG1

Application Details

Application Notes: Immunofluorescence. Immunohistochemistry on Frozen SectionsImmunohistochemistry on Paraffin Embedded Tissue: 1/5-1/10 for 1 h at RT (Aftermicrowave treatment). Immunoblotting (Western): 0.1-0.5 μg/mL. The antibody also reacts with a 44 kD degradation fragment of Synaptopodin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL distilled water

Buffer: Final Solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

References for anti-SYNPO antibody (ABIN112223)

Tan, Rak-Raszewska, Skovorodkin, Vainio: "Mouse Embryonic Stem Cell-Derived Ureteric Bud Progenitors Induce Nephrogenesis." in: Cells, Vol. 9, Issue 2, (2021) (PubMed).

Xu, Chen, Dong, Meyuhas, Chen: "Phosphorylation of ribosomal protein S6 mediates compensatory renal hypertrophy." in: Kidney international, Vol. 87, Issue 3, pp. 543-56, (2015) (PubMed).

Yates, Papakrivopoulou, Long, Goggolidou, Connolly, Woolf, Dean: "The planar cell polarity gene Vangl2 is required for mammalian kidney-branching morphogenesis and glomerular maturation." in: Human molecular genetics, Vol. 19, Issue 23, pp. 4663-76, (2010) (PubMed).

Câmpean, Karpe, Haas, Atalla, Peters, Rupprecht, Liebner, Acker, Plate, Amann: "Angiopoietin 1 and 2 gene and protein expression is differentially regulated in acute anti-Thy1.1 glomerulonephritis." in: American journal of physiology. Renal physiology, Vol. 294, Issue 5, pp. F1174-84, (2008) (PubMed).

Lechler, Wu, Bernhardt, Campean, Gastiger, Hackenbeck, Klanke, Weidemann, Warnecke, Amann, Engehausen, Willam, Eckardt, Rödel, Wiesener: "The tumor gene survivin is highly expressed in adult renal tubular cells: implications for a pathophysiological role in the kidney." in: The American journal of pathology, Vol. 171, Issue 5, pp. 1483-98, (2007) (PubMed).

Target Details for SYNPO

Target: SYNPO (Synaptopodin (SYNPO))

Alternative Name: Synaptopodin / SYNPO (SYNPO Products)

Background: Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data), in mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (the difference might be attributed to posttranslational modifications).Synonyms: KIAA1029

Gene ID: 11346, 9606

UniProt: Q8N3V7