KRT8 antibody

Catalog No. ABIN112173

Product Details anti-KRT8 Antibody

Target: KRT8 (Keratin 8 (KRT8))

Reactivity: Human, Rat, Mouse, Rabbit, Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT8 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: M20 reacts with glandular epithelial cells (endocrine and exocrine) as well as mesothelial cells of the digestive, respiratory and urogenital tract and most adenocarcinomas derived from these cells. The M20 epitope has been mapped to amino acids 353-367 on the alpha helical rod domain of Cytokeratin 8 (See Reference 10).

Purification: Purified

Immunogen: Keratin isolated from the Human breast carcinoma cell line MCF-7

Clone: M20

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: 1/100-1/1000Immunocytochemistry. Flow Cytometry. Immunohistochemistry on Frozen Tissues: 1/100-1/200 with Avidin-biotinylatedHorseradish peroxidase complex ABC as detection reagent.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the lyophilized antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. When reconstituted the antibody is stable for one month at 2-8 °C.

Target Details for KRT8

Target: KRT8 (Keratin 8 (KRT8))

Alternative Name: Cytokeratin 8 (KRT8 Products)

Background: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.Synonyms: CK8, CYK8, Cytokeratin endo A, Cytokeratin-8, K8, KRT8, Keratin, Keratin-8, type II cytoskeletal 8

Gene ID: 3856

NCBI Accession: NP_002264

UniProt: P05787