Macrophages antibody

Catalog No. ABIN112015

Product Details

Target: Macrophages

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognises an epitope of a membrane protein which is associated with a receptor for glycoconjugates (Sialoadhesin). Antigen Distribution: Isolated Cells: TRPM-3 is negative with Monocytes and Granulocytes. Tissue Sections: TRPM-3 stains marginal zone macrophages and marginal metallophils of the spleen strongly. It rarely reacts with cordal Macrophages in the red pulp and interdigitating cells in the periarterial lymphatic sheet of the spleen. In lymph nodes, only sinus Macrophages express the antigen. Moreover, it is expressed on macrophages in the synovial membrane, mucosa and submucosa of the gastrointestinal tract, twisted spindle-shaped macrophages of the omentum and on macrophages in the dermis and subcutaneous tissue. It is absent from macrophages in the thymus, Peyer's patches and from Langerhans cells in the skin.

Cross-Reactivity (Details): Species reactivity (tested):Rat (Macrophages).Negative in Human, Mouse, Hamster, Guinea Pig, Goat and Rabbit.

Purification: Affinity Chromatography.

Immunogen: Peritoneal Macrophages.

Clone: TRPM-3

Isotype: IgG1

Application Details

Application Notes: Suitable for Immunohistochemistry on Frozen Sections: 0.25-0.5 μg/mL (1/400-1/800) when using an ABC amplification system. Suggested Positive Control: Rat Spleen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2, with 0.01 % Thimerosal as preservative and 10 mg/mL BSA as stabilizer.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: Macrophages

Background: TRPM-3 is a specific cell surface marker useful for the identification of tissue fixed rat macrophages and macrophage subpopulations. It is particularly suitable for phenotyping macrophages of lymphoid organs, where it reacts with marginal zone macrophages and with marginal metallophils. It is also an interesting marker for studying autoimmune diseased tissues. TRPM-3 recognises an epitope of a membrane protein which is associated with a receptor for glycoconjugates (sialoadhesin).Synonyms: Macrophage marker