MHC Class II Antigen I Ak,d,b,q,r antibody (Biotin)

Catalog No. ABIN111988

Product Details

Target: MHC Class II Antigen I Ak,d,b,q,r

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

No Cross-Reactivity: Human

Cross-Reactivity (Details): Species reactivity (tested):Mouse (Cells expressing MHC class II antigens).

Purification: Affinity Chromatography

Immunogen: Murine thymic reticulum Remarks: Antigen / Epitope: MHC Class II antigens are heterodimers consisting of one alpha-chain (31-34 kDa) and one beta-chain (26-29 kDa). The epitope has not been further characterized.

Clone: ER-TR3

Isotype: IgG2b

Application Details

Application Notes: Immunohistochemistry on Frozen Sections: 4 μg/mL (1/100). Suggested Positive Control: Mouse spleen. Does not react on routinely processed paraffin sections. Has been reported to work in Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.4 mg/mL

Buffer: Stock Solution contains PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer with no preservative

Preservative: Without preservative

Handling Advice: This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C

Storage Comment: Store at +4 °C. DO NOT FREEZE. This product should be stored undiluted.

Target Details

Target: MHC Class II Antigen I Ak,d,b,q,r

Alternative Name: MHC Class II (I-A K,b,d,q,r)

Background: MHC Class II antigens are heterodimers consisting of one alpha chain (31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR3, ER-TR2, ER-TR1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the Human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.