SFTPD antibody (Conformational Domain) (Biotin)

Catalog No. ABIN111975

Product Details anti-SFTPD Antibody (Biotin)

Target: SFTPD (Surfactant Protein D (SFTPD))

Binding Specificity: Conformational Domain

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SFTPD antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: II E11 recognizes a conformational epitope of SP-D. No cross reactivity with rat SP-A. Antigen distribution: Tissue sections: alveolar type II cells, alveolar macrophages, two types of Clara cells SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells stain negative. No reaction on rat skin, colon, kidney and liver.

Immunogen: Purified rat surfactant protein D from bronchoalveolar lavage

Clone: IIE11

Isotype: IgG2b

Application Details

Application Notes: Immunohistochemistry on frozen sections (1 μg/mL, 1: 400). Immunohistochemistry on paraffin sections (1: 100 freshly prepared for staining rat tissues. Fixation: Acetone 5 min 4 C, paraformaldehyde / paraffin. Tissue pretreatment: microwave treatment 2 x 5 min at 750 W). Western Blot. Suggested positive control: Rat lung.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.5 mL distilled water.

Concentration: 0.4 mg/mL

Buffer: Phosphate buffered saline pH 7.2, (PBS), 5 mg/mL bovine serum albumin (BSA) as a stabilizer, no preservative.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store (in aliquots) at -20 °C for longer.

References for anti-SFTPD antibody (Biotin) (ABIN111975)

Bräuer, Kindler, Jäger, Sel, Nölle, Pleyer, Ochs, Paulsen: "Detection of surfactant proteins A and D in human tear fluid and the human lacrimal system." in: Investigative ophthalmology & visual science, Vol. 48, Issue 9, pp. 3945-53, (2007) (PubMed).

Target Details for SFTPD

Target: SFTPD (Surfactant Protein D (SFTPD))

Alternative Name: SFTPD (SFTPD Products)

Background: Surfactant protein D (SP-D) is a Ca2+-dependent carbohydrate-binding protein and is structurally similar to other C-type mammalian lectins, such as conglutinin and SP-A. It has a molecular size of approximately 43 kDa in its reduced state, 620 kDa in non-dissociating conditions. SP-D enhances the production of oxygen radicals by rat alveolar macrophages and regulates some actions of SP-A, which is the most abundant surfactant protein. SP-D is synthesized and secreted by alveolar epithelial type II cells.Synonyms: COLEC7, Collectin-7, Lung surfactant protein D, PSP-D, PSPD, Pulmonary surfactant-associated protein D, SFTP4, SP-D

Gene ID: 25350

NCBI Accession: NP_037010

UniProt: P35248