SFTPD antibody (Conformational Domain)

Catalog No. ABIN111974

Product Details anti-SFTPD Antibody

Target: SFTPD (Surfactant Protein D (SFTPD))

Binding Specificity: Conformational Domain

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SFTPD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: SPDE recognizes a conformational epitope of SP-D. Clone SPDE is a reclone from clone IIE11, with identical reactivity. Antigen Distribution on Tissue Sections: Alveolar type II cells, alveolar macrophages, two types of Clara cells SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells stain negative. No reaction on rat skin, colon, kidney and liver.

Purification: Affinity Chromatography.

Immunogen: Purified Rat Surfactant protein D from bronchoalveolar lavage.

Clone: IIE11 (SPDE)

Isotype: IgG2b

Application Details

Application Notes: Immunohistochemistry on Frozen Sections: 2 μg/mL (1/250)Does not react on routinely processed paraffin sections. Recommneded Positive Control: Rat lung. Has been described to work in Western blots.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.5 mL distilled water (= 0.5 mg/mL Stock Solution).

Concentration: 0.5 mg/mL

Buffer: Stock solution contains PBS, pH 7.2 with 0.09 % Sodium Azide as preservative and 5 mg/mL BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8 °C for up to one month. For long term storage, aliquot and freeze unused portion at -20 °C in volumes appropriate for single usage. Avoid freeze/thaw cycles. Avoid prolonged exposure to light.

References for anti-SFTPD antibody (ABIN111974)

Bräuer, Kindler, Jäger, Sel, Nölle, Pleyer, Ochs, Paulsen: "Detection of surfactant proteins A and D in human tear fluid and the human lacrimal system." in: Investigative ophthalmology & visual science, Vol. 48, Issue 9, pp. 3945-53, (2007) (PubMed).

Target Details for SFTPD

Target: SFTPD (Surfactant Protein D (SFTPD))

Alternative Name: SFTPD (SFTPD Products)

Background: Surfactant Protein D (SP-D) is a Ca2+-dependent carbohydrate-binding protein and is structurally similar to other C-type mammalian lectins, such as conglutinin and SP-A. It has a molecular size of approximately 43 kDa in its reduced state, 620 kDa in non-dissociating conditions. SP-D enhances the production of oxygen radicals by rat alveolar macrophages and regulates some actions of SP-A, which is the most abundant surfactant protein. SP-D is synthesized and secreted by alveolar epithelial type II cells.Synonyms: COLEC7, Collectin-7, Lung surfactant protein D, PSP-D, PSPD, Pulmonary surfactant-associated protein D, SFTP4, SP-D

Gene ID: 6441

NCBI Accession: NP_003010

UniProt: P35247