Mucin-Like Carcinoma Antigen (MCA) antibody

Catalog No. ABIN111966

Product Details

Target: Mucin-Like Carcinoma Antigen (MCA)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Affinity Chromatography.

Immunogen: Breast carcinoma cell lines.

Clone: B-12

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on: Frozen sections: 0.5 μg/mL (1/200). Paraffin sections: 4 μg/mL (1/25), Proteinase K pretreatment for antigen retrievalrecommended. Suggested positive control: Human uterus. Antigen Distribution: In contrast to MCA producing tumors, the b-12 related antigen is onlylocated at the MCA producing sites such as glandular cell surfaces or glandular tubuli. InMCA producing tumors, where cells become disorganized, the b-12 antigen is secreted intostromal tissue and blood vessels. (See Table 1. )
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum. Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Incubate the tissue section with proteinase K for 7 min. 3. Wash in distilled water4. Block endogenous peroxidase5. Wash in PBS6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber8. Wash in PBS9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber10. Wash in PBS11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 12. Wash in PBS13. Counterstain with Mayer's hemalum.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.1 mg/mL

Buffer: PBS, buffer pH 7.2 with 0.05 % Sodium Azide as preservative and 2 mg/mL BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: Mucin-Like Carcinoma Antigen (MCA)

Background: Synonyms: Marker for Mucin Producing Cells