CEACAM5 antibody

Catalog No. ABIN111951

Product Details anti-CEACAM5 Antibody

Target: CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CEACAM5 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: C19 is useful to detect CEA in malignant adenocarcinoma, notably in the gastro-intestinal tract. Clinical relevance is also found in breast-, lung-, prostatic-,ovarian- and other carcinomas of endodermal origin (stomach, liver, pancreas). Contrary to some other antibodies, C19 stains granulocytes in blood smears and tonsils, as well as epithelial cells.

Purification: Affinity chromatography.

Clone: C19

Isotype: IgG1

Application Details

Application Notes: Suitable for Immunohistochemistry on paraffin embedded sections (0.1 μg/mL, 1/4000). Microwave pretreatment forantigen retrieval is recommended. Suggested positive control: Human colon carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.4 mg/mL

Buffer: PBS, pH 7.2 with 10 mg/mL bovine serum albumin (BSA) as a stabilizer and 0.01 % thimerosal as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for CEACAM5

Target: CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))

Alternative Name: CD66e / CEACAM5 (CEACAM5 Products)

Background: CEA is a glycoprotein of 180 kDa m.w. It is a normal constituent of the glycocalix of embryonic endodermal epithelium and is produced mainly in the mucosa of the normal colon.Synonyms: CEA, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, Meconium antigen 100

Gene ID: 1048

NCBI Accession: NP_004354

UniProt: P06731