TNFRSF1B antibody

Catalog No. ABIN111942

Product Details anti-TNFRSF1B Antibody

Target: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNFRSF1B antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G.

Immunogen: Partially purified preparations of TNF binding proteins from U937 cells.

Clone: Utr1

Isotype: IgG1

Application Details

Application Notes: Immunohistology: 2-5 μg/mL on Cryostat Sections (2 % Periodate-lysine-paraformaldehydeor acetone for fixation). Flow Cytometry: 10 μg/mL. Immunoprecipitation. Western blots: 30 μg/mL on non-reduced SDS PAGE, the epitope is sensitive to reduction,may need enrichment by Immunoprecipitation. Functional Assay: This antibody utr1 is useful for studying biological effects ofTNF-Receptor p75 in vitro where it inhibits the binding of radiolabelled TNF to human cellsexpressing the p75 TNF receptor. In order to completely block TNF binding to the cellsurface, 10 μg/mL of utr1 are required. utr1 itself may have an agonistic effect in assaysmeasuring cytotoxicity, fibroblast growth or IL-6 secretion.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum

Restrictions: For Research Use only

Handling

Concentration: 2.0 mg/mL (by Absorbanece at 280 nm)

Buffer: PBS, pH 7.2, None, None

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and in aliquots at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after two weeks at 4 °C.

Target Details for TNFRSF1B

Target: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Alternative Name: CD120b / TNFR2 (TNFRSF1B Products)

Background: Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF receptor superfamily along with FAS antigen and CD40. TNF receptors I and II are 55 and 75 kDa members, respectively, of a family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 4-1BB, which are characterized by cysteine rich motifs in the extracellular domain. TNF Receptor II (p75, CD120b) is present on most cell types (including monocytes, endothelial cells, Langerhans cells, and macrophages) and is considered to play a role in cell stimulation by TNF alpha. TNF Receptor II molecule is shown to be responsible for stimulation of activated T lymphocytes by TNF alpha.Synonyms: TNF-R2, TNFBR, TNFRSF1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor type II, p80 TNF-alpha receptor

Gene ID: 9606

UniProt: P20333

Pathways: NF-kappaB Signaling, Apoptosis, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Ubiquitin Proteasome Pathway