TNFRSF1B antibody (Biotin)

Catalog No. ABIN111941

Product Details anti-TNFRSF1B Antibody (Biotin)

Target: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNFRSF1B antibody is conjugated to Biotin

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogen: Partially purified preparations of TNF binding proteins

Clone: Utr1

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on Frozen Sections: 1 μg/mL (1/400) andImmunohistochemistry on Paraffin Sections: 10 μg/mL (1/40). Pretreatment not necessary. Recommended Positive Control: Human tonsil. Has also been described to work in FACS, Western Blots and Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer’s hemalum.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.5 mL distilled water.

Concentration: 0.4 mg/mL

Buffer: PBS, pH 7.2, containing 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody undiluted Prior to and After reconstitution at 2-8 °C. DO NOT FREEZE!.

Target Details for TNFRSF1B

Target: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Alternative Name: CD120b / TNFR2 (TNFRSF1B Products)

Background: Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF receptor superfamily along with FAS antigen and CD40. TNF receptors I and II are membrane glycoproteins and they are from the family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 4-1BB, which are characterized by cysteine rich motifs in the extracellular domain. TNF Receptor II (p75, CD120b) is present on most cell types (including monocytes, endothelial cells, Langerhans cells, and macrophages) and is considered to play a role in cell stimulation by TNF alpha. TNF Receptor II molecule is shown to be responsible for stimulation of activated T lymphocytes by TNF alpha.Synonyms: TNF-R2, TNFBR, TNFRSF1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor type II, p80 TNF-alpha receptor

Gene ID: 9606

UniProt: P20333

Pathways: NF-kappaB Signaling, Apoptosis, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Ubiquitin Proteasome Pathway