TNFRSF1A antibody

Catalog No. ABIN111939

Product Details anti-TNFRSF1A Antibody

Target: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNFRSF1A antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G.

Immunogen: Partially purified preparation of TNF binding protein from HL-60 cells.

Clone: Htr9

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 10 μg/mL. Immunohistochemistry on Frozen Sections: 2-5 μg/mL on Cryostat Sections. Concentrations < 25 μg/mL may give non specific staining of discrete cells. This putativecross reactivity with nuclear (?) antigens cannot be abrogated by pre- or coincubation withTNF. Fixation: Acetone 5 min 4 °C or 2 % paraformaldehyde-lysine sodium periodate.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by Absorbance at 280 nm)

Buffer: PBS, pH 7.2 without stabilizers and preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at 2-8 °C and reconstituted (in aliquots) at -20 °C.

Target Details for TNFRSF1A

Target: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Alternative Name: CD120a / TNFR1 (TNFRSF1A Products)

Background: Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF Receptor superfamily along with FAS antigen and CD40. TNF Receptors I and II are 55 and 75 kDa members, respectively, of a family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 41BB, which are characterized by cysteine rich motifs in the extracellular domain. While TNF Receptor I and TNF Receptor II share 28 % sequence homology in the extracellular domains, their intracellular domains lack sequence homology, suggesting that they differ in their internal signal transduction pathways. TNF Receptor I contains an approximately 80 amino acid death domain near its carboxy terminus capable of transmitting an apoptotic signal through its interaction with TRADD (TNF Receptor I associated death domain protein), and subsequent interactions with FADD. TNF Receptor I can also activate the transcription factor NFkB via TRAF2 (TNF Receptor associated factor 2). The cytoplasmic domain of TNF Receptor I can directly interact with Jak kinase, thereby activating the JAK/STAT signal transduction cascade. TNF Receptor I is expressed by virtually all nucleated mammalian cells, including hepatocytes, monocytes and neutrophils, cardiac muscle cells, endothelial cells, and CD34 + hematopoietic progenitors. Both TNF alpha and TNF beta bind to TNF Receptor I.Synonyms: TNF-R1, TNF-RI, TNFR-I, Tnfrsf1a, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor type I, p55, p60

Gene ID: 9606

UniProt: P19438

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Hepatitis C, Ubiquitin Proteasome Pathway