CD163 antibody (Biotin)

Catalog No. ABIN111916

Product Details anti-CD163 Antibody (Biotin)

Target: CD163

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD163 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Clone 5C6 FAT recognizes CD163, a membrane glycoprotein on human monocytes and macrophages which is expressed in intermediate and late inflammatory stages. Antigen distribution on isolated cells: Monocytes, particularly after dexamethasone treatment or after 2-5 days in culture. Does not react with lymphocytes, granulocytes or platelets. Antigen Distribution Tissue Sections: Positive staining can be observed in the skin (histiocytes), gut, Kupffer cells, few alveolar macrophages, a major population of macrophages in the placenta, varying degrees of macrophages in inflamed tissues, including tumorous tissue depending on the inflammatory stage. Red pulp, but not white pulp macrophages of the spleen, andcortical macrophages of the thymus are detected. Macrophages in the synovialis of patients with rheumatoid arthritis. In alveolar macrophages and in Kupffer cells a double staining can be observed with monoclonal antibody BM4022 (clone 25F9) which is not the case in other tissues. Isolated Cells: Monocytes, particularly after dexamethasone treatment or after 2-5 days in culture. Does not react with lymphocytes, granulocytes or platelets. Negative In Pig.

Purification: Affinity Chromatography

Immunogen: Human CD163. The epitope has not been further characterized.

Clone: 5C6-FAT

Isotype: IgG1

Application Details

Application Notes: Tested for immunohistochemistry (IHC) and ELISA, has been described to work in FACS andWestern blots. Approximate working dilution for IHC: Frozen sections: 0.5 μg/mL (1: 400)Paraffin sections: 1 μg/mL, Proteinase K pretreatment for antigen retrieval recommended. Suggested positive control: Human placenta.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.2 mg/mL

Buffer: Stock Solution containing PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as a preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

References for anti-CD163 antibody (Biotin) (ABIN111916)

Bir, Calli-Demirkan, Tufan, Akbulut, Satiroglu-Tufan: "Apoptotic cell death and its relationship to gastric carcinogenesis." in: World journal of gastroenterology : WJG, Vol. 13, Issue 23, pp. 3183-8, (2007) (PubMed).

Zaba, Cardinale, Gilleaudeau, Sullivan-Whalen, Suárez-Fariñas, Suárez Fariñas, Fuentes-Duculan, Novitskaya, Khatcherian, Bluth, Lowes, Krueger: "Amelioration of epidermal hyperplasia by TNF inhibition is associated with reduced Th17 responses." in: The Journal of experimental medicine, Vol. 204, Issue 13, pp. 3183-94, (2007) (PubMed).

Target Details for CD163

Target: CD163

Alternative Name: CD163 (CD163 Products)

Synonyms: DMBT1 antibody, CD163 antibody, CD163v2 antibody, CD163v3 antibody, M130 antibody, MM130 antibody, deleted in malignant brain tumors 1 protein-like antibody, CD163 molecule antibody, CD163 antigen antibody, LOC426820 antibody, CD163 antibody, Cd163 antibody

Background: CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and is upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. CD163-positive cells include skin histiocytes, Kupffer cells, spleen macrophages of the red pulp, and some thymus macrophages. The antigen is also found abundantly in human term placenta, and regularly in acute and chronic inflammatory lesions.Synonyms: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130

Gene ID: 9332

NCBI Accession: NP_004235

UniProt: Q86VB7