CD33 antibody
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- Target See all CD33 Antibodies
- CD33
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD33 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- The CD33 antibody is valuable for distinguishing acute myeloid from lymphoblastic leukaemias. CD33 is expressed on monocytes in peripheral blood. In the bone marrow, CD33 antigen is found on granulocyte and macrophage precursors, and is absent from pluripotent stem cells.
- Cross-Reactivity (Details)
- Species reactivity (tested):Human.
- Purification
- Affinity Chromatography
- Clone
- MY9
- Isotype
- IgG2b
- Top Product
- Discover our top product CD33 Primary Antibody
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anti-CD33 (CD33) (AA 101-200) antibody
CD33 Reactivity: Human, Mouse FACS, WB, ELISA, IHC (fro), IF (p), IF (cc), ICC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-CD33 (CD33) (AA 15-237) antibodyCD33 Reactivity: Human FACS, ELISA, IHC Host: Mouse Monoclonal 2C6B7 unconjugated
anti-CD33 (CD33) (AA 18-240) antibodyCD33 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-CD33 (CD33) (AA 49-259) antibodyCD33 Reactivity: Human WB, IF, IHC Host: Rabbit Polyclonal unconjugated
anti-CD33 (CD33) (AA 261-364) antibodyCD33 Reactivity: Human FACS, WB, ELISA, IHC (fro), IF (p), IF (cc), IHC (p) Host: Rabbit Polyclonal unconjugated
anti-CD33 (CD33) (AA 1-364) antibodyCD33 Reactivity: Human WB, ELISA, RNAi Host: Mouse Monoclonal 2D12-G4 unconjugated
anti-CD33 (CD33) antibodyCD33 Reactivity: Human, Non-Human Primate FACS, WB, IP, IHC (fro), ICC, Func, CyTOF Host: Mouse Monoclonal WM53 unconjugated
anti-CD33 (CD33) antibodyCD33 Reactivity: Human, Non-Human Primate FACS, WB, IP, IHC (fro), ICC, CyTOF Host: Mouse Monoclonal WM53 unconjugated
anti-CD33 (CD33) antibody (Biotin)CD33 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal HIM3-4 Biotin
anti-CD33 (CD33) antibody (FITC)CD33 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal WM53 FITC
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- Application Notes
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Immunohistochemistry (IHC) on frozen sections: 4 μg/mL (1: 100). FACS: 10 l stock solution to label 10^6 cells. Immunoprecipitation. Suggested positive control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore with 0.5 mL distilled water.
- Concentration
- 0.4 mg/mL
- Buffer
- PBS, pH 7.2, 0.05 % (v/v) Kathon CG, 5 mg/mL BSA
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C
- Storage Comment
- Store the antibody at 2-8 °C after reconstitution.
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- Target
- CD33
- Alternative Name
- CD33 / SIGLEC3 (CD33 Products)
- Synonyms
- SIGLEC-3 antibody, SIGLEC3 antibody, p67 antibody, Siglec-3 antibody, gp67 antibody, CD33 molecule antibody, CD33 antigen antibody, CD33 antibody, Cd33 antibody
- Background
- CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias. Identification of human Monocytes (bright) and Granulocytes (dim) expressing the 67 kDa M.W. surface antigen. CD33 is a 67 kDa single chain transmembrane glycoprotein that belongs to the sialoadhesin family.Synonyms: Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67
- Gene ID
- 945
- NCBI Accession
- NP_001763
- UniProt
- P20138
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