CD14 antibody

Catalog No. ABIN111907

Product Details anti-CD14 Antibody

Target: CD14

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Monoclonal antibody X-8 prevents the Lipopolysaccharide Binding Protein (LBP) independent activation of cells via soluble CD14 by blocking the binding of CD14. BM4034 is specific for Human CD14 on monocytes, some populations of macrophages and granulocytes. The epitope has not been further characterized.

Purification: Affinity Chromatography.

Immunogen: Human Monocytes.

Clone: X-8

Isotype: IgG2b

Application Details

Application Notes: Immunohistochemistry on Frozen Sections: 2-4 μg/mL (1/50-1/100). Does not react on routinely processed Paraffin Sections. Recommended Positive Control: Human placenta. Has been described to work in FACS (post fixation is recommended).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.5 mL distilled water (= Stock solution).

Concentration: 0.2 mg/mL

Buffer: PBS pH 7.2 containing 10 mg/mL BSA as stabilizer and 0.01 % Thimerosal as preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody undiluted Prior to and Following Reconstitution at 2-8 °C. DO NOT FREEZE!

Target Details for CD14

Target: CD14

Alternative Name: CD14 (CD14 Products)

Background: CD14 is a 53'000 - 55'000 Mr glycoprotein strongly expressed on the surface of monocytes and weakly on the surface of granulocytes. It is anchored to the surface by linkage to glycosyl phosphatidylinositol (GPI). CD14 functions as a receptor for endotoxin and is the major receptor involved in the lethal response to both endotoxin and Gram-negative bacteria. In addition to membrane CD14, soluble forms of CD14 can be detected in serum and tissue culture supernatants of cells transfected with CD14.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

Gene ID: 929, 9606

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades