Macrophages antibody (Biotin)

Catalog No. ABIN111880

Product Details

Target: Macrophages

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The antibody is also suitable for staining macrophages from bronchial lavage fluids and similar techniques. It is very useful for macrophage phenotyping, particularly for the classification of late inflammatory stages (together with 27E10, RM 3/1, and G 16/1) in tissue sections and in smears, for the characterization of tumorous tissues and the monitoring of macrophage cell cultures. This antibody reacts with human mature macrophages and subpopulation of macrophages of rhesus monkey,pig alveolar macrophages and Kupffer cells. The antigen is an 86 kDa protein (unreduced conditions), probably a glycoprotein on the cell surface and within the cytoplasm of mature macrophages. It is stable to formaldehyde fixation and paraffin embedding. Enzyme digestion is recommended. Antigen Distribution on Isolated cells and Tissue Sections: Absent on freshly isolated monocytes and other blood cells, present on 40 - 50 % of human monocytes after 6-7 day culture, also positive on some melanoma and carcinoma cell lines. Kupffer cells, histiocytes (skin), macrophages of the thymus, in the germinal centres of lymph nodes and spleen, in mamma carcinoma, melanoma, osteocarcinoma and gastric cancer, excema, sarcoidosis, BCG granuloma, synovial lining cells, tuberculoid leprosy: no expression in lepramatous leprosy.

Cross-Reactivity (Details): Species reactivity (tested):Human (mature Macrophages and Monocytes).Other: subpopulation of Macrophages in Rhesus monkey,reactive with Pig alveolar Macrophages and Kupffer cells.

Purification: Affinity Chromatography.

Clone: 25F9

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on: Frozen and paraffin embedded sections: 2 μg/mL (1/200)Paraffin sections: 4 μg/mL (1/100). Proteinase K pretreatment for antigen retrieval isrecommended. Suggested positive control: Human tonsil. Antigen Distribution on Isolated cells and Tissue sections: Absent on freshly isolatedmonocytes and other blood cells, present on 40 - 50 % of human monocytes after 6-7 dayculture, also positive on some melanoma and carcinoma cell lines. Kupffer cells, histiocytes (skin), macrophages of the thymus, in the germinal centres oflymph nodes and spleen, in mamma carcinoma, melanoma, osteocarcinoma and gastriccancer, excema, sarcoidosis, BCG granuloma, synovial lining cells, tuberculoid leprosy: noexpression in lepramatous leprosy.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Buffer: PBS buffer, pH 7.2 with 0.01 % Thimerosal as preservative and 10 mg/mL BSA as stabilizer.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody at 2-8° C (undiluted).

Target Details

Target: Macrophages

Background: 25F9 is associated with well-differentiated tissue macrophages both in normal and diseased tissues independently of the presence or absence of inflammation. The antigen is a 86 kDa membrane protein, the epitope has not been further characterized.Synonyms: Macrophage marker