Granulocytes antibody

Catalog No. ABIN111863

Product Details

Target: Granulocytes

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purification: Affinity Chromatography

Immunogen: Peritoneal cells.

Clone: RK-4

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on Frozen Sections: 1.25 μg/mL (1/400). Immunohistochemistry on Paraffin Sections (After enzyme digestion). Suggested Positive Control: Rat spleen, blood smears. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Staining procedure for frozen ice-cold acetone-fixed tissue sections: (The whole procedure is performed at room temperature)

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water to make stock solution.

Concentration: 0.5 mg/mL IgG

Buffer: Stock Solution contains PBS, pH 7.2 with 5 mg/mL BSA as stabilizer and 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Target Details

Target: Granulocytes

Background: Granulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells. Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules, Eosinophils have prominent granules that stain readily with the acid dye eosin, and Basophils have prominent granules that stain readily basic (non acidic) dyes.Synonyms: Granulocyte cells