This antibody is useful for the detection of the F4/80 antigen on major subpopulations of resident tissue macrophages. The antigen expression increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny. This clone is the only Macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas and has been shown to be a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model. Antigen Distribution Tissue Sections: The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. In complete Freund's adjuvant induced granulomas the antigen is expressed by inflammatory macrophages, but is absent from epitheloid cells. Isolated Cells: expressed in vitro on over 80 % of M-CSF stimulated bone marrow derived macrophages, after a few days of culture. It is absent from granulocytes, lymphocytes and thrombocytes.Antigen Distribution
Cross-Reactivity (Details)
Species reactivity (tested):Mouse. Also stains Human Heart Macrophages.
Purification
Affinity Chromatography
Immunogen
Cultured Macrophages. Remarks: The antigen/epitope is a 125 kDa extracellular membrane protein sensitive to 2-Mercaptoetanol.
EMR1
Reactivity: Mouse, Human, Rat, Dog
WB, IHC (p)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Immunohistochemistry on Frozen Sections: 0.4 μg/mL (1/500). Immunohistochemistry on Paraffin Sections: 4 μg/mL (1/50). Proteinase K pretreatment forantigen retrieval is recommended. Suggested Positive Control: Mouse spleen. Has been described to work in FACS. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol
Protocol with Frozen, ice-cold Acetone-Fixed Sections: (The whole procedure is performed at room temperature)1. Wash in PBS
Restrictions
For Research Use only
Reconstitution
Restore by adding 0.5 mL distilled water.
Concentration
0.2 mg/mL
Buffer
Stock Solution contains PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer and 0.01 % Kathon as a preservative
Preservative
Kathon CG
Precaution of Use
This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Braunger, Leimbeck, Schlecht, Volz, Jägle, Tamm: "Deletion of ocular transforming growth factor ? signaling mimics essential characteristics of diabetic retinopathy." in: The American journal of pathology, Vol. 185, Issue 6, pp. 1749-68, (2015) (PubMed).
Lemmermann, Krmpotic, Podlech, Brizic, Prager, Adler, Karbach, Wu, Jonjic, Reddehase, Adler: "Non-redundant and redundant roles of cytomegalovirus gH/gL complexes in host organ entry and intra-tissue spread." in: PLoS pathogens, Vol. 11, Issue 2, pp. e1004640, (2015) (PubMed).
Ilves, Palomäki, Vippola, Lehto, Savolainen, Savinko, Alenius: "Topically applied ZnO nanoparticles suppress allergen induced skin inflammation but induce vigorous IgE production in the atopic dermatitis mouse model." in: Particle and fibre toxicology, Vol. 11, pp. 38, (2014) (PubMed).
Stangl, Gehrmann, Riegger, Kuhs, Riederer, Sievert, Hube, Mocikat, Dressel, Kremmer, Pockley, Friedrich, Vigh, Skerra, Multhoff: "Targeting membrane heat-shock protein 70 (Hsp70) on tumors by cmHsp70.1 antibody." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 2, pp. 733-8, (2011) (PubMed).
Spyridonidou, Fousteris, Antonia, Chatzianastasiou, Papapetropoulos, Nikolaropoulos: "Tricyclic indole and dihydroindole derivatives as new inhibitors of soluble guanylate cyclase." in: Bioorganic & medicinal chemistry letters, Vol. 19, Issue 16, pp. 4810-3, (2009) (PubMed).
Kimura, Miyashita, Suzuki, Kobayashi, Watanabe, Sonoda, Ohta, Fujiwara, Shimosegawa, Sato: "Distinctive localization and opposed roles of vasohibin-1 and vasohibin-2 in the regulation of angiogenesis." in: Blood, Vol. 113, Issue 19, pp. 4810-8, (2009) (PubMed).
Kakehashi, Nishioku, Tsukuba, Kadowaki, Nakamura, Yamamoto: "Differential regulation of the nature and functions of dendritic cells and macrophages by cathepsin E." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 9, pp. 5728-37, (2007) (PubMed).
Torzewski, Shaw, Han, Shortal, Lackner, Witztum, Palinski, Tsimikas: "Reduced in vivo aortic uptake of radiolabeled oxidation-specific antibodies reflects changes in plaque composition consistent with plaque stabilization." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 24, Issue 12, pp. 2307-12, (2004) (PubMed).
F4/80 protein binds to Macrophages from different sites including the peritoneal cavity, lung, spleen and thymus, to blood monocytes and to macrophages derived from bone marrow precursors in culture. F4/80 is not expressed by lymphocytes or polymorphonuclear cells.Synonyms: Cell surface glycoprotein EMR1, EMR1 hormone receptor, Emr1, Gpf480