CD68 antibody (PE)

Catalog No. ABIN111833

Product Details anti-CD68 Antibody (PE)

Target: CD68 (CD68 Molecule (CD68))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD68 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: This antibody recognises CD68.

Purification: Affinity Chromatography on Protein A

Immunogen: Rat spleen cells. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.

Clone: ED1

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Neat - 1/10, use 10 μL of the suggested working dilution to label 10^6 cellsin 100 μL, Results maybe enhanced using membrane permeabilisation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL distilled water

Buffer: PBS, 0.09 % Sodium Azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Should this product contain a precipitate we recommend microcentrifugation before use.

References for anti-CD68 antibody (PE) (ABIN111833)

Schneider, Meusel, Renker, Bauer, Holzinger, Roeder, Wanner, Gekle, Sauvant: "Low-dose indomethacin after ischemic acute kidney injury prevents downregulation of Oat1/3 and improves renal outcome." in: American journal of physiology. Renal physiology, Vol. 297, Issue 6, pp. F1614-21, (2009) (PubMed).

Target Details for CD68

Target: CD68 (CD68 Molecule (CD68))

Alternative Name: CD68 (CD68 Products)

Background: The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.Synonyms: Gp110, Macrophage marker, Macrosialin

Gene ID: 287435

NCBI Accession: NP_001026808

UniProt: Q4FZY1