CD68 antibody (FITC)
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- Target See all CD68 Antibodies
- CD68 (CD68 Molecule (CD68))
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Reactivity
- Rat, Cow
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD68 antibody is conjugated to FITC
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Application
- Flow Cytometry (FACS)
- Specificity
- This antibody recognises CD68.
- Purification
- Affinity Chromatography on Protein A.
- Immunogen
- Rat Spleen cells. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line.
- Clone
- ED1
- Isotype
- IgG1
- Top Product
- Discover our top product CD68 Primary Antibody
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- Application Notes
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Flow Cytometry: Use 10 μL of neat antibody to labenl 10^6 cells in 100 μL. Results may beenhanced using membrane permeabilisation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/mL
- Buffer
- PBS containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- CD68 (CD68 Molecule (CD68))
- Alternative Name
- CD68 (CD68 Products)
- Background
- The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.Synonyms: Gp110, Macrophage marker, Macrosialin
- Gene ID
- 287435
- NCBI Accession
- NP_001026808
- UniProt
- Q4FZY1
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