The antibody reacts with a 180 kDa isoform of CD56 (NCAM) expressed in leukocytes. It has been suggested that the antibody could react with rhesus monkey lymphocytes. Reactivity with other NCAM isoforms has not been tested.
Cross-Reactivity (Details)
Species reactivity (tested):Human, Non-Human Primates
Flow Cytometry: Use 10 μL to label 10^6 cells or 100 μL whole blood or blood or 10^6 cells ina suspension. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Buffer
PBS containing 15 mM Sodium Azide and 0.2 % (w/v) high-grade BSA (Protease free) as a stabilizing agent, 15 mM Sodium Azide, 0.2 % (w/v) high-grade BSA (Protease free)
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody prior to reconstitution at 2-8 °C. Following reconstitution store (in aliquots) at -20 °C.
CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of immunoglobulin family serving as adhesive molecule which is ubiquitously expressed in nervous system, usually as 120 kDa, 140 kDa or 180 kDa isoform, and it is also found on T cells and NK cells. Polysialic modification results in reduction of CD56-mediated cell adhesion and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumours and ovarian granulosa cell tumours.Synonyms: N-CAM-1, NCAM, NCAM-1, Neural cell adhesion molecule 1