CD42b antibody (APC)

Catalog No. ABIN1112142

Product Details anti-CD42b Antibody (APC)

Target: CD42b (GP1BA) (Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD42b antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Characteristics: Monoclonal Mouse Anti-Human CD42b APC is recommended for use in flow cytometry for identification of platelets and megakaryocytes.

Clone: HIP1

Isotype: IgG1

Application Details

Application Notes: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

Comment:

Allophycocyanin (Europa Bioproducts, Ely, Cambridge- Prozyme).

Sample Preparation:

1. Centrifuge tube of freshly drawn EDTA blood (600rpm or 75xg, 20') 2. Remove platelets (top layer) and wash twice over in PBS and resuspend in PBS. 3. Add 20 µl of CD42b APC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 4. The recommended negative control is a non-reactive PE-conjugated antibody of the same isotype. 5. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 6. Analyse on a flow cytometer.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

Preservative: Sodium azide

Precaution of Use: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Storage: 4 °C

Target Details for CD42b

Target: CD42b (GP1BA) (Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA))

Alternative Name: CD42b (GP1BA Products)

Background: The antibody is directed against the CD42b antigen, platelet glycoprotein gp1bA, that serves as a receptor for Von Willebrand factor (vWF) and as a high affinity thrombin receptor. AK2 immunblots a band of 130kDa on SDS gels of platelet lysates and gives surface immunofluorescence with fixed or unfixed platelets. The antigen is absent in very low levels on platelets with the Bernard-Soulier syndrome.