CD20 antibody (Biotin)

Catalog No. ABIN1112084

Product Details anti-CD20 Antibody (Biotin)

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD20 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Characteristics: Mouse Monoclonal Anti-Human CD20 BIOTIN is recommended for use in flow cytometry for identification of B cells in peripheral blood, lymph nodes, spleen, tonsil and bone marrow..

Clone: LT20

Isotype: IgG1

Application Details

Application Notes: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 10 µl, 1/100, of the suggested working dilution to label 10^6 cells.

Comment:

BIOTIN( Sigma).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing Antibody Stabilizer.

Preservative: Sodium azide

Precaution of Use: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Storage: 4 °C

References for anti-CD20 antibody (Biotin) (ABIN1112084)

Cragg, Morgan, Chan, Morgan, Filatov, Johnson, French, Glennie: "Complement-mediated lysis by anti-CD20 mAb correlates with segregation into lipid rafts." in: Blood, Vol. 101, Issue 3, pp. 1045-52, (2003) (PubMed).

Orfao, Ciudad, Gonzalez, Lopez, del Mar Abad, Paz Bouza, Cruz, Gomez Alonso, San Miguel: "Flow cytometry in the diagnosis of cancer." in: Scandinavian journal of clinical and laboratory investigation. Supplementum, Vol. 221, pp. 145-52, (1995) (PubMed).

Target Details for CD20

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Alternative Name: CD20 (MS4A1 Products)

Background: This antibody recognizes the 33-37 kDa molecular weight membrane-embedded non- glycosylated phosphoprotein made up of two polypeptide chains. CD20 is present on all normal B cells in peripheral blood, lymph nodes, spleen, tonsil and bone marrow. The antigen is also present on most B cell lines and on non-T ALL and CLL cells.