CD15, CD34 antibody (FITC, R-PE)

Catalog No. ABIN1112064

Product Details

Target: CD15, CD34

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: FITC, R-PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Characteristics: Monoclonals Mouse Anti-Human CD15/CD34, are recommended for use in flow cytometry for identification of myelomonocytic cells and myeloid progenitor cells in erythrocyte lysed whole blood, based on cell-surface antigen expression. Changes in the CD15/ CD34 phenotype may be useful in studying normaland malignant myeloid differentiation.

Clone: MCS-1-581

Isotype: IgG3, IgG1

Application Details

Application Notes: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells. It can be used to study expression of the myeloid leukemias.

Comment:

Fluorescein Isothiocyanate (FITC) and R-Phycoerythrin (R-PE).

Sample Collection: 1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD15 FITC/CD34 PE and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive FITC-conjugated antibody of the same isotype, and a non-reactive PE-conjugated antibody of the same Isotype. 4. Incubate in the dark at room temperature at 4°C for 15 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The conjugates are provided in liquid form in buffer containing Antibody Stabilizer solution, PBS 20 mM, pH 7,2. Fluorochromes: Fluorescein Isothiocyanate (FITC) and R-Phycoerythrin (R-PE).

Preservative: Sodium azide

Precaution of Use: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Storage: 4 °C

Target Details

Target: CD15, CD34