Histone H1 antibody

Catalog No. ABIN3155738

Product Details anti-Histone H1 Antibody

Target: Histone H1

Reactivity: Arabidopsis thaliana, Nicotiana tabacum, Wheat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Cross-Reactivity: Arabidopsis thaliana

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: Lathyrus sativus, Phaseolus vulgaris, Pisum sativum, Solanum lycopersicum, Vicia faba

Characteristics: Expected / apparent Molecular Weight of the Antigene: 15 / 17 kDa
Histone 1 (H1) located in nuclei, incorporated into chromatin.

Purification: serum

Immunogen: native H1 protein purified from Nicotiana tabaccum (H1A, H1B H1C,D,E,F)

Application Details

Application Notes: 1 : 5000 with standard ECL (WB), 1: 100 - 1: 500 (ICC)

Comment:

Protocol for isolation of cytosolic and nuclear fractions can be found here.Images of immunolocalization work with this antibody, can be found in a reference below.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for Histone H1

Target: Histone H1

Alternative Name: Histone H1 (H1) (Histone H1 Products)

Synonyms: H10 antibody, H1FV antibody, H1 histone family member 0 antibody, H1F0 antibody

Background: Histone 1 (H1) is a protein located in nuclei, incorporated into chromatin.

Molecular Weight: expected: 15 kDa, apparent: 17 kDa