Vimentin antibody
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- Target See all Vimentin (VIM) Antibodies
- Vimentin (VIM)
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Reactivity
- Human, Mouse, Rat
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This Vimentin antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF)
- Sequence
- CAVLRpSSVP GV
- Specificity
- This antibody recognizes the site-specific phosphorylation of vimentin at Ser71.
- Cross-Reactivity (Details)
- Species reactivity (tested):Human (U251), mouse (NIH/3T3), rat (3Y1-B)
- Purification
- Protein G agarose
- Immunogen
- KLH conjugated phospho-peptide PV71
- Clone
- TM71
- Isotype
- IgG2a
- Top Product
- Discover our top product VIM Primary Antibody
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS containing 50 % glycerol, pH 7.2. No preservative is contained.
- Preservative
- Without preservative
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store (in aliquots) at -20 °C.
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- Target
- Vimentin (VIM)
- Alternative Name
- Vimentin (VIM Products)
- Background
- Components of intermediate filaments provide information on the origin of vertebrate cells. Antibody to vimentin can be used as to identify the vimentin subclass of intermediate filaments. Vimentin is a ~58 kDa, widely expressed protein that thought to be involved mainly in structural processes, such as wound healing. Scientists have also recently determined that activated human macrophages secrete vimentin into the extracellular space, and overproduction of vimentin has been associated with cellular senescence.Synonyms: VIM
- Gene ID
- 7431
- NCBI Accession
- NP_003371
- UniProt
- P08670
- Pathways
- Caspase Cascade in Apoptosis
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