SYT1 antibody

Catalog No. ABIN1109184

Product Details anti-SYT1 Antibody

Target: SYT1 (Synaptotagmin I (SYT1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SYT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Specificity: This antibody reacts with Synaptotagmin 1 and 2 (58 kDa/60 kDa) on Western blotting. Synaptotagmin 1 has a predicted molecular weight of 47 kDa but it migrates at ~65 kDa. It is reported that this clone (1D12) recognizes rat synaptotagmin residues (99-104 aa) in the reference number 17).

Cross-Reactivity (Details): Species reactivity (tested):Rat

Purification: Protein A agarose

Immunogen: Chicken brain synaptic membrane

Clone: 1D12

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 50 % glycerol, pH 7.2, without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store (in aliquots) at -20 °C.

Target Details for SYT1

Target: SYT1 (Synaptotagmin I (SYT1))

Alternative Name: Synaptotagmin-1 (SYT1 Products)

Background: Synaptotagmins are a family of at least 15 Ca2+-binding, membrane-trafficking proteins that act as calcium sensors regulating neurotransmitter release. Synaptotagamins are expressed in both vesicular and plasma membranes where they are involved in lipid rearrangements required during synaptic vesicle fusion. Synaptotagmins also interact with calcium channels, sodium channels, b-SNAP, neurexins, and other neural proteins in the absence of Ca2+, suggesting a role in presynaptic functions. Synaptotagmins exhibit differential expression, with synaptotagmins 1-5 expressed primarily in neural and endocrine tissues. Northern blots in murine brain tissue suggest synaptotagmin 1 is primarily expressed in rostral brain regions but has low expression in regions related to motor functions.Synonyms: SVP65, SYT, SYT2, Synaptotagmin I, Synaptotagmin II, Synaptotagmin1, Synaptotagmin2, Synonyms: SYT1, SytI, SytII, p65

Gene ID: 6857

NCBI Accession: NP_001129277

UniProt: P21579

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport