CD68 antibody

Catalog No. ABIN1106532

Product Details anti-CD68 Antibody

Target: CD68 (CD68 Molecule (CD68))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD68 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This Monoclonal Antibody ED1 is useful for detecting Rat monocytes and macrophages and isolated dendritic (veiled) cells in the blood. The antibody recognises a single chain glycoprotein of 90-100 kDa. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen recognised by ED1 has many characteristics in common with Mouse macrosialin and Human CD68. Antigen, Epitope: CD68, ED1 recognises a 92 kDa cytoplasmic protein. The epitope has not been further characterized. Antigen Distribution: The antigen is found on 90 % of monocytes in the peripheral blood. It is also expressed by 98 % of isolated dendritic (veiled) cells.

Cross-Reactivity (Details): Species reactivity (tested):Rat: Monocytes Macrophages, Dendritic Cells.

Purification: Affinity Chromatography

Clone: ED1

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for CD68

Target: CD68 (CD68 Molecule (CD68))

Alternative Name: CD68 (CD68 Products)

Background: The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.Synonyms: Gp110, Macrophage marker, Macrosialin

Gene ID: 287435