BST1 antibody

Catalog No. ABIN1105911

Product Details anti-BST1 Antibody

Target: BST1 (Bone Marrow Stromal Cell Antigen 1 (BST1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BST1 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: This antibody reacts with CD157 antigen. This antibody reacts with RASV5-5 cells, and does not react with a healthy donor-derived nonsupportive cell line, NFSV1-1.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein A agarose

Immunogen: RA-derived BM stromal cell line RASV5-5

Clone: RF3

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Prepare a stock solution by dissolving the lyophilized antibody in 100 μL of distilled water. After reconstitution, the IgG concentration will be 1 mg/mL.

Buffer: PBS ( pH 7.2), 0.09 % NaN3, 1 % sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store (in aliquots) at -20 °C.

Target Details for BST1

Target: BST1 (Bone Marrow Stromal Cell Antigen 1 (BST1))

Alternative Name: CD157 / BST1 (BST1 Products)

Background: In the bone marrow (BM) hematopoietic microenvironment, stromal cells play crucial roles. Stromal cells secrete a variety of cytokines and express cell surface molecules that regulate the growth of hematopoietic cells. BM stromal cell lines derived from patients with rheumatoid arthritis (RA) have augmented ability to support the growth of a murine pre-B cell line, DW34. BST-1 (bone marrow stromal cell antigen-1) is expressed on BM stromal cell lines and is responsible for an augmented ability to support pre-B cell line growth. BST-1 is also expressed by RA-derived synovial cell lines, a myelomonocytic cell lines, and HUVEC, suggesting that BST-1 has other functional roles than just supporting pre-B cell growth.Synonyms: ADP-ribosyl cyclase 2, BST-1, Bone marrow stromal antigen 1, Cyclic ADP-ribose hydrolase 2, cADPr hydrolase 2

Gene ID: 683

NCBI Accession: NP_004325

UniProt: Q10588