GFP antibody (Alkaline Phosphatase (AP))
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- Target See all GFP Antibodies
- GFP (Green Fluorescent Protein (GFP))
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Reactivity
- Aequorea victoria
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This GFP antibody is conjugated to Alkaline Phosphatase (AP)
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Specificity
- Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215, recombinant GFP from Aequorea victoria) by both Western blot and ELISA. No reaction is seen against RFP.
- Purification
- Protein A purified
- Sterility
- Sterile filtered
- Immunogen
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The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Type of Immunogen: Fusion protein - Clone
- 9F9-F9
- Isotype
- IgG
- Top Product
- Discover our top product GFP Primary Antibody
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- Application Notes
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Approved: ELISA, IHC (1:200 - 1:1000), WB (1:500 - 1:2500)
Usage: Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with a polyclonal anti-GFP antibody as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Aequorea victoria
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.05 M Tris chloride, 0.15 M sodium chloride, 1 mM magnesiuim chloride, 0.1 mM zinc chloride, pH 8.0, 50 % glycerol, 1 % BSA, 0.01 % sodium azide, sterile filtered
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not freeze.
- Storage
- 4 °C
- Storage Comment
- Store at 4°C. Do not freeze.
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- Target
- GFP (Green Fluorescent Protein (GFP))
- Alternative Name
- GFP (GFP Products)
- Synonyms
- green fluorescent protein antibody, gfp antibody
- Background
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Name/Gene ID: GFP
Synonyms: GFP - UniProt
- P42212
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