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GFP antibody (Biotin)

GFP Reactivity: Aequorea victoria WB, ELISA, IHC Host: Goat Polyclonal Biotin

Catalog No. ABIN1101735

Datasheet as PDF

Target See all GFP Antibodies

GFP (Green Fluorescent Protein (GFP))
Reactivity
174

15

11

9
Aequorea victoria
Host
64

62

34

13

9

5

2

1
Goat
Compatible Secondaries
Clonality
113

72
Polyclonal
Conjugate
81

12

11

10

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is conjugated to Biotin
Application
147

75

46

33

27

25

23

21

14

13

13

12

12

5

4

4

2

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-biotin and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.

Purification

Immunoaffinity purified

Immunogen

Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Type of Immunogen: Fusion protein

Isotype

IgG

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GFP Reactivity: Aequorea victoria FACS, IM Host: Goat Polyclonal FITC

anti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea victoria WB, IP, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

Approved: ELISA, IHC (1:1000 - 1:5000), WB (1:2000 - 1:10000)

Usage: GFP Antibody is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment

Target Species of Antibody: Aequorea victoria

Restrictions

For Research Use only
Format

Liquid

Reconstitution

deionized water

Concentration

Lot specific

Buffer

0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 1 % BSA, 0.01 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

avoid freeze thaw cycles.

Storage

4 °C,-20 °C

Storage Comment

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Target

GFP (Green Fluorescent Protein (GFP))

Alternative Name

GFP (GFP Products)

Synonyms

green fluorescent protein antibody, gfp antibody

Background

Name/Gene ID: GFP

Synonyms: GFP

UniProt

P42212