FITC antibody (HRP)

Catalog No. ABIN1099691

Product Details anti-FITC Antibody (HRP)

Target: FITC (Fluorescein Isothiocyanate (FITC))

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This FITC antibody is conjugated to HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Dot Blot (DB)

Specificity: This antiserum has not been tested for cross-reactivity with other fluorescent compounds.

Characteristics: The reactivity of the antiserum is directed to the FITC molecule as tested in direct binding enzyme immunoassay, ELISA and immunoblotting.

IgG concentration is 0.4 mg/mL. Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. No foreign proteins added.

Immunogen: Highly purified fluorescein isothiocyanate isomer 1.

Clone: NI 239

Application Details

Application Notes: The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:100 to 1:500, in ELISA from 1:1,000 upwards, in Western blotting from 1:2,000 upwards. These data should be interpreted as general recommendations only.

Comment:

Physical form: Horseradish peroxidase conjugated purified mouse IgG1 lyophilized from a solution in phosphate buffered saline (pH 7.2).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Handling Advice: The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day.

Storage: 4 °C

Target Details for FITC

Target: FITC (Fluorescein Isothiocyanate (FITC))

Alternative Name: FITC (FITC Products)

Target Type: Chemical

Background: To enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to FITC. The phenomenon of a weak reaction of a monoclonal antibody is e.g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the FITC hapten-anti-hapten system makes it a valuable alternative to the biotin- avidin system.