ICOSLG antibody (Extracellular Domain)

Catalog No. ABIN108723

Product Details anti-ICOSLG Antibody

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Binding Specificity: Extracellular Domain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICOSLG antibody is un-conjugated

Application: Flow Cytometry (FACS), Cell-ELISA (cELISA)

Specificity: GM13C1 recognises the extracellular domain of ICOSL transiently expressed on the cell surface of transfected BOSC cells as well as the native protein on antigen presenting cells (see reference).

Purification: Protein G

Immunogen: genetic immunisation with cDNA encoding human ICOSL (extracellular domain)

Clone: GM-13C1

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1.2 µg/10^6 cells
CELISA: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.

Comment:

Synonyms: ICOS ligand

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C

Storage Comment: short term: 2 °C - 8 °C, long term: -20 °C

Target Details for ICOSLG

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Alternative Name: ICOSL (ICOSLG Products)

Background: The inducible co-stimulator receptor (ICOS) is a member of the CD28 receptor family that is expressed on T cells. ICOS ligand (ICOSL) has been identified as a ligand that binds to ICOS, but not to CD28 or CTLA4. GM13C1 was generated by genetic immunisation and recognises the extracellular domain of ICOSL.

UniProt: O75144

Pathways: Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation