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p53 antibody (AA 108-370)

Name: p53 antibody (AA 108-370)
SKU: ABIN1078633
Price: 265.2 USD
Availability: InStock

TP53 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

Western Blotting (WB) image for anti-Tumor Protein P53 (TP53) (AA 108-370) antibody (ABIN1078633) (p53 antibody (AA 108-370))

Used in DAB staining on fromalin fixed paraffin-embedded Kidney tissue (p53 antibody (AA 108-370))

IHC

Knockout Varification: Lane 1: Wild-type MCF7 cell lysate; Lane 2: TP53 knockout MCF7 cell lysate; Predicted MW: 24,30,33,34,38,39,44kDa Observed MW: 46kDa Primary Ab: 1µg/ml Rabbit Anti-Human TP53 Antibody Second Ab: 0. (p53 antibody (AA 108-370))

4 images

(1 reference)

Catalog No. ABIN1078633

Datasheet as PDF

Target See all p53 (TP53) Antibodies

p53 (TP53) (Tumor Protein P53 (TP53))
Binding Specificity
43

40

37

30

29

29

26

26

24

22

20

20

19

19

18

18

16

15

13

9

8

8

8

7

7

6

6

6

6

6

6

6

5

5

4

4

4

4

4

4

4

4

4

4

3

3

3

3

3

3
AA 108-370
Reactivity
825

252

208

104

47

33

31

18

10

9

7

6

6

3

3

3

2

2

2

2

2

1

1

1
Human
Host
624

218

6

2

1
Rabbit
Compatible Secondaries
Clonality
559

291

1
Polyclonal
Conjugate
478

38

31

19

18

13

13

13

13

13

13

13

13

13

12

11

11

11

11

11

11

11

11

5

5

5

5

5

5

2

2

2

2

2

2

2

1

1

1

1

1

1
This p53 antibody is un-conjugated
Application
662

298

247

200

200

148

124

122

119

64

59

32

18

16

13

12

9

9

8

6

4

3

3

2

2

2

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose

Polyclonal Antibody to Tumor Protein p53 (P53)

Specificity

The antibody is a rabbit polyclonal antibody raised against P53. It has been selected for its ability to recognize P53 in immunohistochemical staining and western blotting.

Cross-Reactivity

Rat

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen

Recombinant Tumor Protein p53 (P53) corresdonding to Gly108~Lys370 with N-terminal His Tag

Isotype

IgG

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anti-Tumor Protein P53 (TP53) (pSer33) antibody
TP53 Reactivity: Human, Mouse, Rat WB, IHC Host: Rabbit Polyclonal unconjugated

Application Notes

Western blotting: 0.3-2 μg/mL
Immunohistochemistry: 5-20 μg/mL
Immunocytochemistry: 5-20 μg/mL
Optimal working dilutions must be determined by end user.

Comment

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative

ProClin

Precaution of Use

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice

Avoid repeated freeze-thaw cycles.

Storage

4 °C,-20 °C

Storage Comment

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date

24 months
Chen, Liu, Liu, Li, Cheng: "Transcription factor HBP1: A regulator of senescence and apoptosis of preadipocytes." in: Biochemical and biophysical research communications, Vol. 517, Issue 2, pp. 216-220, (2020) (PubMed).
Target

p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name

Tumor Protein p53 (TP53 Products)

Background

TP53, LFS1, TRP53, Li-Fraumeni Syndrome, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

Pathways

p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect