TYMS antibody

Catalog No. ABIN107488

Product Details anti-TYMS Antibody

Target: TYMS (Thymidylate Synthetase (TYMS))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This TYMS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This antibody was purified from rabbit serum after repeated immunizations with recombinant human Thymidylate Synthase (36 kDa) produced in E.coli.
Immunogentype:Recombinant

Isotype: IgG

Application Details

Application Notes: THYMIDYLATE SYNTHASE antibody has been tested for use in ELISA, immunoprecipitation, immunofluorescence microscopy, immunohistochemistry and immunoblotting.  The antibody recognizes the expected additional band corresponding to the ternary complex of hTS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR.  This event occurs in most human breast, colorectal, gastric, head and neck carcinomas.  The antibody recognizes the 36,000 MW hTS.  Reactivity in other immunoassays is unknown.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for TYMS

Target: TYMS (Thymidylate Synthetase (TYMS))

Alternative Name: Thymidylate Synthase (TYMS Products)

Target Type: Viral Protein

Background: Synonyms: d TMP synthase antibody, EC 2.1.1.45 antibody, HsT422 antibody, MGC88736 antibody

Gene ID: 7298

UniProt: P04818

Pathways: Mitotic G1-G1/S Phases