Streptavidin antibody (HRP)

Catalog No. ABIN107094

Product Details anti-Streptavidin Antibody (HRP)

Target: Streptavidin

Reactivity: Streptomyces avidinii

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Streptavidin antibody is conjugated to HRP

Application: ELISA

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: Streptavidin [Streptomyces avidinii]
Immunogentype:Native

Isotype: IgG

Application Details

Application Notes: This product has been assayed against 1.0 ug of Streptavidin in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) for 30 minutes at room temperature. Optimal concentrations in immunoassyas should be determined by researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Gentamicin sulfate

Handling Advice: Do NOT add Sodium Azide!

Storage: 4 °C

Target Details for Streptavidin

Target: Streptavidin

Abstract: Streptavidin Products

Background: Anti-streptavidin antibody detects Streptavidin, a 60 kDa protein purified from the bacterium Streptomyces avidinii. Streptavidin is a homo-tetrameric protein having a very high affinity for biotin (vitamin B7). Streptavidin bound biotin has a dissociation constant (Kd) on the order of about 10^−14 mol/L. Streptavidin and biotin are used extensively in molecular biology and bionanotechnology due to the resistance of streptavidin-biotin complex to organic solvents, denaturants (e.g. guanidinium chloride), detergents (e.g. SDS, Triton), proteolytic enzymes, and extremes of temperature and pH.
Synonyms: HRP