CD34 antibody (C-Term)
-
- Target See all CD34 Antibodies
- CD34
-
Binding Specificity
- C-Term
-
Reactivity
- Human
-
Host
- Goat
-
Clonality
- Polyclonal
-
Conjugate
- This CD34 antibody is un-conjugated
-
Application
- Immunofluorescence (IF), ELISA
- Purpose
- CD34-T
- Sequence
- SPTGERLELE P
- Specificity
- This antibody is expected to recognize reported isoform CD34-T (NP_001020280.1) only.
- Cross-Reactivity
- Human, Mouse
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-SPTGERLELEP, from the of the protein sequence according to NP_001764.1.
- Isotype
- IgG
- Top Product
- Discover our top product CD34 Primary Antibody
-
-
- Application Notes
-
Western Blot: Preliminary testing showed bands at approx 85+90 kDa in Human Peripheral Blood Mononucleocytes lysate (calculated MW of 35.0 kDa according to NP_001764.1). The observed MW correspond to glycosylated forms, routinely observed by other sources.
Peptide ELISA: antibody detection limit dilution 1:128000.
- Comment
-
Immunofluorescence: Strong expression of the protein seen in the plasma membranes and nuclei of Jurkat and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: A customer reported weak staining of Jurkat cel
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
- Target
- CD34
- Alternative Name
- CD34 (CD34 Products)
- Synonyms
- AU040960 antibody, CD34 molecule antibody, CD34 antigen antibody, CD34 antibody, Cd34 antibody
- Background
- CD34, CD34 Molecule, CD34 antigen, OTTHUMP00000034733, OTTHUMP00000034734, hematopoietic progenitor cell antigen CD34, CD34-T
- Gene ID
- 947, 12490
- NCBI Accession
- NP_001764
-