OspB antibody

Catalog No. ABIN1043770

Product Details

Target: OspB

Reactivity: Borrelia burgdorferi

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Specificity: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi OspB protein. A BLAST analysis was used to suggest cross-reactivity with OspB from B. burgdorferi, afzelii, spielmanii, and garinii sources based on 100% homology with the immunizing sequence, and with B. valaisiana based on 99% homology. Cross-reactivity with OspB from other sources has not been determined.

Characteristics: OspB, is one of the major Outer Surface Proteins of the outer membrane of Borrelia burgdorferi, which is composed of various unique outer surface proteins (Osp) that have been characterized (OspA through OspF). The Osp proteins are lipoproteins anchored by N-terminally attached fatty acid molecules to the membrane. They are presumed to play a role in virulence, transmission, or survival in the tick. Two of the major surface Ag of Borrelia burgdorferi, the 31-kDa OspA and 34-kDa OspB proteins, show a high degree of sequence similarity, are encoded by a 49-kb plasmid and share a common promoter, and are coordinately transcribed. OspA, OspB, and OspD are expressed by B. burgdorferi residing in the gut of unfed ticks, suggesting that they promote the persistence of the spirochete in ticks between blood meals. OspB has a contributing role in the adherence of B. burgdorferi to the tick gut. B. burgdorferi has the ability to vary its surface proteins in response to immune attack.

Purification: purified

Sterility: Sterile filtered

Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi OspB protein.
Immunogen Type: RecombinantProtein

Isotype: IgG

Application Details

Application Notes: Suggested dilutions:
ELISA : 1:13,000
Western Blot : 1:1,000
Other Dilution: User Optimized

Comment:

Gene Name: ospB, locus BB_A16

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 3 months

References

Neelakanta, Li, Pal, Liu, Beck, DePonte, Fish, Kantor, Fikrig: "Outer surface protein B is critical for Borrelia burgdorferi adherence and survival within Ixodes ticks." in: PLoS pathogens, Vol. 3, Issue 3, pp. e33, (2007) (PubMed).

Liang, Caimano, Radolf, Fikrig: "Borrelia burgdorferi outer surface protein (osp) B expression independent of ospA." in: Microbial pathogenesis, Vol. 37, Issue 1, pp. 35-40, (2004) (PubMed).

Haake: "Spirochaetal lipoproteins and pathogenesis." in: Microbiology (Reading, England), Vol. 146 ( Pt 7), pp. 1491-504, (2000) (PubMed).

Target Details

Target: OspB

Background: OspB, is one of the major Outer Surface Proteins of the outer membrane of Borrelia burgdorferi, which is composed of various unique outer surface proteins (Osp) that have been characterized (OspA through OspF). The Osp proteins are lipoproteins anchored by N-terminally attached fatty acid molecules to the membrane. They are presumed to play a role in virulence, transmission, or survival in the tick. Two of the major surface Ag of Borrelia burgdorferi, the 31-kDa OspA and 34-kDa OspB proteins, show a high degree of sequence similarity, are encoded by a 49-kb plasmid and share a common promoter, and are coordinately transcribed. OspA, OspB, and OspD are expressed by B. burgdorferi residing in the gut of unfed ticks, suggesting that they promote the persistence of the spirochete in ticks between blood meals. OspB has a contributing role in the adherence of B. burgdorferi to the tick gut. B. burgdorferi has the ability to vary its surface proteins in response to immune attack.
Synonyms: Outer surface protein B, Borrelia burgdorferi OspB

Gene ID: 1194340

UniProt: P17739