B-Phycoerythrin antibody

Catalog No. ABIN1043760

Supplier Product No.: 200-401-099

Product Details anti-B-Phycoerythrin Antibody

Target: B-Phycoerythrin (Phycoerythrin, B-)

Reactivity: Red algae (Porphyridium cruentum)

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)

Supplier Product No.: 200-401-099

Supplier: Rockland

Purpose: B-Phycoerythrin Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Phycoerythrin [Porphyridium creuentum].

Characteristics: Synonyms: rabbit anti-B-Phycoerythrin Antibody, rabbit anti B-Phycoerythrin, rabbit anti Phycoerythrin

Purification: Anti-B-Phycoerythrin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Phycoerythrin [Porphyridium creuentum]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-B-Phycoerythrin antibody has been tested by ELISA and dot blot and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:20,000 - 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-B-Phycoerythrin antibody (ABIN1043760)

Firl, Degn, Padera, Carroll: "Capturing change in clonal composition amongst single mouse germinal centers." in: eLife, Vol. 7, (2018) (PubMed).

Target Details for B-Phycoerythrin

Target: B-Phycoerythrin (Phycoerythrin, B-)

Alternative Name: B-Phycoerythrin (B-Phycoerythrin Products)

Background: Background: Anti-B-Phycoerythrin Antibody detects the B-PE phycoerythrin protein. Phycoerythrin is a red protein from the light-harvesting phycobiliprotein family, present in cyanobacteria, and red algae. Phycoerythrin is organized in a hexameric structure of alpha and beta chains, covalently binding chromophores called phycobilins. R-Phycoerythrin and B-Phycoerythrin are among the brightest biological fluorescent dyes and are commonly used as a fluorescent dye in many assays and is frequently conjugated to antibodies as a reporter molecule. The spectral properties of B-Phycoerythrin are absorption at 545 nm and emission at 572 nm.

UniProt: P11392