CD34 antibody (Extracellular Domain)

Catalog No. ABIN1042500

Product Details anti-CD34 Antibody

Target: CD34

Binding Specificity: Extracellular Domain

Reactivity: Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Cell-ELISA (cELISA)

Characteristics: Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells

Purification: Protein G

Immunogen: genetic immunisation with cDNA encoding the extracellular domain of ovine CD34

Clone: EQ-8D11-C1

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: 1.2 µg/10^6 cells
Immunofluorescence: 1 µg/10^6 cells
CELISA: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C

Storage Comment: short term: 2 °C - 8 °C, long term: -20 °C

Target Details for CD34

Target: CD34

Alternative Name: Ovine CD34 (CD34 Products)

Background: CD34 is a type I membrane protein with an apparent MW of approximately 115 kDa. It is a putative adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. CD34 is expressed specifically on immature normal human marrow cells, including hematopoietic progenitor cells. It is thought to act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components.