ACR-Modified Keyhole-Lympet Hemocyanine antibody

Catalog No. ABIN1020376

Product Details

Target: ACR-Modified Keyhole-Lympet Hemocyanine

Host: Mouse

Clonality: Monoclonal

Application: Immunohistochemistry (IHC)

Specificity: Specific for ACR-modified protein (especially FDP-lysine type derivative).

Purification: Protein A purified

Clone: 5F6

Isotype: IgG1 kappa

Application Details

Application Notes: Recommended antibody concentration is 0.5-1.0 µg/mL on paraformaldehyde fixed tissue.

Restrictions: For Research Use only

Handling

Format: Frozen

Buffer: 100 µg/mL antibody in 10mM PBS containing 0.1% NaN3 and 0.5% BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References

Sugiyama, Sun, Nishinohara, Fujita, Masuda, Ochi, Takeuchi: "Expressions of lipid oxidation markers, N(ε)-hexanoyl lysine and acrolein in cisplatin-induced nephrotoxicity in rats." in: The Journal of veterinary medical science / the Japanese Society of Veterinary Science, Vol. 73, Issue 6, pp. 821-6, (2011) (PubMed).

Target Details

Target: ACR-Modified Keyhole-Lympet Hemocyanine

Background: Acrolein (ACR) is a representative carcinogenic aldehyde found ubiquitously in the environment and formed endogenously through oxidation reactions, such as lipid peroxidation and myeloperoxidasecatalyzed amino acid oxidation. ACR is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with ACR and lysine residue leads to the formation of numerous numbers of adducts, such as formyl-dehydropiperidino-lysine (FDP-lysine) type derivative. This antibody is specific for the ACR-modified protein, especially FDP-lysine type derivative.